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Additionally it would be highly
2018-11-12
Additionally, it would be highly desirable to manipulate CSCs to improve their survival and direct them efficiently to the myocyte lineage. A recently developed technology to deliver mRNA in vivo shows promise. Modified RNA (modRNA) encoding human vascular endothelial growth factor-A (VEGF-A) improv
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br Introduction The human gallbladder mainly functions to
2018-11-12
Introduction The human gallbladder mainly functions to concentrate and store bile, and like anatomically close-by organs—the liver and the pancreas—is derived from the foregut endoderm during embryonic development (Spence et al., 2009). The extrahepatic biliary tree (comprising extrahepatic bile
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One most recent study first reported the feasibility
2018-11-12
One most recent study first reported the feasibility of using undifferentiated hPSC g-quadruplex for cardiac differentiation in suspension by small molecules modulating Wnt pathway (Kempf et al., 2014). With a fixed differentiation condition without controlling cell aggregate size, the authors show
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Cy3.5 maleimide Supplier br DNA fingerprinting analysis The
2018-11-12
DNA fingerprinting analysis The target regions of genomic DNA were amplified by Polymerase chain reaction (PCR). The PCR was carried out using a Thermocycler type PTC100 (MJ Research Inc) for 35cycles and each cycle contained 96°C for 30s, 56°C for 30s and 72°C for 30s. The following primer seque
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Transplantation assays which measure the potential of cells
2018-11-09
Transplantation assays, which measure the potential of tachykinins under investigation, traditionally were used as the gold standard for characterizing tissue stem cells. However, recent studies in epithelial tissues based on pulse-chase lineage-tracing approaches, which measure the activity of prim
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br Results br Discussion This study describes
2018-11-09
Results Discussion This study describes a simplified protocol for the efficient generation of hiNSCs via direct reprogramming. hiNSC lines grow as colonies on MEF feeder layers. Like hESCs, hiNSCs stain positive for pluripotent transcription factors OCT4, SOX2, and NANOG, but do not express ce
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Our studies also revealed that reduced oxygen content
2018-11-09
Our studies also revealed that reduced oxygen content did not promote the formation of SOX9+/RUNX2+ nodules, in contrast to previous studies showing that hypoxia controls differentiation of prechondrogeneic Flavopiridol hydrochloride partly through regulation of Sox9 gene expression (Amarilio et al
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br Experimental Procedures br Author Contributions br Introd
2018-11-09
Experimental Procedures Author Contributions Introduction Microglia are resident, tissue-specific macrophages that perform several critical roles in development and maintenance of the CNS (Hanisch and Kettenmann, 2007). They arise from primitive CD45+CX3CR1− myeloid progenitors in the yolk
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Detailed description of the process of
2018-11-09
Detailed description of the process of NPC samples fabrication from the carbides of boron, zircon, titanium and molybdenum and typical parameters of the product structure were disclosed in [53–55]. The structural parameters substantially differed for the samples of different origin. Thus, the mean p
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We have previously reported the CM chip that enables precise
2018-11-09
We have previously reported the CM chip that enables precise estimation of rare cell counts in blood including spiked-in cultured cancer liothyronine sodium (Yatsushiro et al., 2010; Yamamura et al., 2012). Although the CM chip produced excellent results in a preclinical study, we considered that pr
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br Conflicts of Interest br Author Contributions br
2018-11-09
Conflicts of Interest Author Contributions Acknowledgments We thank Dr. Smogorzewska for the gift of RA3331 cell lines, Dr. Rosselli for PD20 cells, Dr. N. Gilbert for LINE-1 constructs, and Dr. W. An for the LINE-1-LUC construct. We thank Drs. Dufourt, Cristofari and Gilbert for fruitful
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Development of a vaccine to
2018-11-09
Development of a vaccine to block RBC invasion has proven to be an elusive goal. Much of the effort has been focused on the merozoite surface protein 1 (MSP1) and the apical membrane antigen 1 (AMA1). Multiple preclinical vaccine studies have demonstrated, using growth inhibition assays (GIA), that
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Puromycin The following are the supplementary data related
2018-11-09
The following are the supplementary data related to this article. Funding Sources T.I. is supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) no. 26461257, and Yokohama Foundation for Advancement of Medical Science.
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Electroencephalography EEG and ERPs have high temporal
2018-11-09
Electroencephalography (EEG) and ERPs have high temporal accuracy and have been successfully used to investigate infants’ processing of visual and auditory social information (De Haan, 2007; Trainor, 2010). For example, ERPs show that 4-month-old infants discriminate between their mother\'s voice an
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br Conflict of interest br Acknowledgements This work was
2018-11-09
Conflict of interest Acknowledgements This work was supported by the Economic and Social Research Council (grant number ES/L003155/1). Introduction The attainment of flexible and effective emotion regulation skills during childhood is considered a key developmental task in support of psych
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